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KMID : 0357019960120020159
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Journal of the Korean Vascular Surgery Society
1996 Volume.12 No. 2 p.159 ~ p.166
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The Effect of Peripheral Blood Lymphocyte Products on Human Arterial Smooth Muscle Cell Proliferation
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Abstract
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Prominent histologic features of abdominal aortic aneurysm (AAA) include medial attenuation with elastin lamellar fragmentation, and a decrease in the number of medial smooth muscle cells(SMC). Another striking feature is inflammatory cell
infiltration
localized in the outer media and adventitia; these cells are predominately T-lymphocytes. The pathology suggests that inflammatory mediators may be responsible for vessel wall changes. Therefore, we sought to determine the effect of soluble
products
from stimulated and unstimulated peripheral blood lymphocytes (PBL) on human arterial SMC proliferation in vitro.
SMC cultures were established from enzymatically digested infrarenal aorta of transplant donors. PBL(5×106 cells/ml) were incubated with and without 40 ng/ml phytohemagglutinin(PHA) for 30 minutes, washed, and then incubated for 24 hours. PBL
supernatant (10% volume) was added to SMC cultures. Cellular proliferation was determined by Coulter counting triplicate samples at 24, 48, 72 and 96 hours. Data are expressed as mean±SEM, compared by ANOVA, and differences considered
significant
when
p<0.05.
PBL activation was verified by noting increased levels of IL-2(45%) and IL-10(5-fold) in PHA-stimulated PBL media. SMC proliferation did not differ between unstimulated and PHA-stimulated groups at any time point. Soluble mediators from PBL,
which
are
predominately T-cells, have minimal effect on growth of proliferating SMC. These data do not support a role for T-cell products in the loss of medial SMC in AAA.
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KEYWORD
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